Please use this identifier to cite or link to this item:
https://scidar.kg.ac.rs/handle/123456789/10372
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rajković, Snežana | - |
dc.contributor.author | Ašanin, Darko | - |
dc.contributor.author | Zivkovic, Marija | - |
dc.contributor.author | Djuran, Miloš | - |
dc.date.accessioned | 2021-04-20T15:35:24Z | - |
dc.date.available | 2021-04-20T15:35:24Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0020-1693 | - |
dc.identifier.uri | https://scidar.kg.ac.rs/handle/123456789/10372 | - |
dc.description.abstract | 1H NMR spectroscopy was applied to the study the reactions of [Pt(en)(Me-mal-O,O′)] and [Pt(en)(Me2-mal-O,O′)] complexes (en is ethylenediamine, Me-mal and Me2-mal are bidentate coordinated anions of 2-methylmalonic and 2,2-dimethylmalonic acids, respectively) with N-acetylated Ac-l-Met-Gly and Ac-l-Met-l-His-type peptides (Ac-l-Met-l-His, Ac-l-Met-Gly-l-His-GlyNH2 and Ac-l-Met-Gly-Gly-l- His-Gly). The use of Me-mal and Me2-mal Pt(II) complexes in the above reactions allows convenient monitoring of their biscarboxylate group via methyl peaks by 1H NMR measurements. All reactions were realized at 37°C with equimolar amounts of the Pt(II) complex and the dipeptide at pH 7.40 in 50 mM phosphate buffer in D2O. In all these reactions the ring-opened Me-mal and Me2-mal Pt(II) adducts as an intermediate products were detected in solution for more than 48 h. We found that during this time in the reaction with Ac-l-Met-Gly these monodentate bound malonate ligands have been replaced by water molecule leading to the formation of the corresponding aqua Pt(II)-peptide complex which further promotes the regioselective cleavage of the peptide. However, in the reaction with Ac-l-Met-l-His-type peptides a selective intramolecular replacement of these malonate anions by the N3 imidazole nitrogen atom from histidine residue was occurred. This replacement reaction leads to the formation of the S,N3-macrochelate Pt(II)-peptide complex which was shown as very stable and hydrolytically inactive for more than two weeks. © 2012 Elsevier B.V. All rights reserved. | - |
dc.rights | restrictedAccess | - |
dc.source | Inorganica Chimica Acta | - |
dc.title | <sup>1</sup>H NMR study of the reactions between carboplatin analogues [Pt(en)(Me-mal-O,O′)] and [Pt(en)(Me<inf>2</inf>-mal-O,O′)] and various methionine- and histidine-containing peptides under physiologically relevant conditions | - |
dc.type | article | - |
dc.identifier.doi | 10.1016/j.ica.2012.11.004 | - |
dc.identifier.scopus | 2-s2.0-84871374181 | - |
Appears in Collections: | Faculty of Medical Sciences, Kragujevac Faculty of Science, Kragujevac Institute for Information Technologies, Kragujevac |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
PaperMissing.pdf Restricted Access | 29.86 kB | Adobe PDF | View/Open |
Items in SCIDAR are protected by copyright, with all rights reserved, unless otherwise indicated.