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Назив: Galectin-3 deficiency accelerates high-fat diet-induced obesity and amplifies inflammation in adipose tissue and pancreatic islets
Аутори: Pejnović, Nada
Pantic, Jelena
Jovanovic I.
Radosavljevic, Gordana
Milovanovic, Marija
Nikolić, Ivana
Zdravkovic, Nemanja
djukic, aleksandar
Arsenijevic, Nebojsa
Lukic, Miodrag
Часопис: Diabetes
Датум издавања: 1-јун-2013
Сажетак: Obesity-induced diabetes is associated with low-grade inflammation in adipose tissue and macrophage infiltration of islets. We show that ablation of galectin-3 (Gal-3), a galactoside-binding lectin, accelerates high-fat diet-induced obesity and diabetes. Obese LGALS3-/- mice have increased body weight, amount of total visceral adipose tissue (VAT), fasting blood glucose and insulin levels, homeostasis model assessment of insulin resistance, and markers of systemic inflammation compared with diet-matched wild-type (WT) animals. VAT of obese LGALS3-/- mice exhibited increased incidence of type 1 T and NKT lymphocytes and proinflammatory CD11c+CD11b+ macrophages and decreased CD4 +CD25+ FoxP3+ regulatory T cells and M2 macrophages. Pronounced mononuclear cell infiltrate, increased expression of NLRP3 inflammasome and interleukin-1β (IL-1β) in macrophages, and increased accumulation of advanced glycation end products (AGEs) and receptor for AGE (RAGE) expression were present in pancreatic islets of obese LGALS3 -/- animals accompanied with elevated phosphorylated nuclear factor-κB (NF-κB) p65 and mature caspase-1 protein expression in pancreatic tissue and VAT. In vitro stimulation of LGALS3-/- peritoneal macrophages with lipopolysaccharide (LPS) and saturated fatty acid palmitate caused increased caspase-1-dependent IL-1β production and increased phosphorylation of NF-κB p65 compared with WT cells. Transfection of LGALS3-/- macrophages with NLRP3 small interfering RNA attenuated IL-1β production in response to palmitate and LPS plus palmitate. Obtained results suggest important protective roles for Gal-3 in obesity-induced inflammation and diabetes. © 2013 by the American Diabetes Association.
URI: https://scidar.kg.ac.rs/handle/123456789/9487
Тип: Article
DOI: 10.2337/db12-0222
ISSN: 00121797
SCOPUS: 84878239208
Налази се у колекцијама:Faculty of Medical Sciences, Kragujevac
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