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Назив: Enrichment of live unlabelled cardiomyocytes from heterogeneous cell populations using manipulation of cell settling velocity by magnetic field
Аутори: Sofla A.
Andjelkovic-Cirkovic B.
Hsieh A.
Miklas, Jason
Filipovic, Nenad
Radisic, Milica
Часопис: Biomicrofluidics
Датум издавања: 4-феб-2013
Сажетак: The majority of available cardiomyocyte markers are intercellular proteins, limiting our ability to enrich live cardiomyocytes from heterogeneous cell preparations in the absence of genetic labeling. Here, we describe enrichment of live cardiomyocytes from the hearts of adult mice in a label-free microfluidic approach. The separation device consisted of a vertical column (15 mm long, 700 μm diameter), placed between permanent magnets resulting in a field strength of 1.23 T. To concentrate the field at the column wall, the column was wrapped with 69 μm diameter nickel wire. Before passing the cells through the column, the cardiomyocytes in the cell suspension had been rendered paramagnetic by treatment of the adult mouse heart cell preparation with sodium nitrite (2.5 mM) for 20 min on ice. The cell suspension was loaded into the vertical column from the top and upon settling, the non-myocytes were removed by the upward flow from the column. The cardiomyocytes were then collected from the column by applying a higher flow rate (144 μl/min). We found that by applying a separation flow rate of 4.2 μl/min in the first step, we can enrich live adult cardiomyocytes to 93% ± 2% in a label-free manner. The cardiomyocytes maintained viability immediately after separation and upon 24 h in culture. © 2013 American Institute of Physics.
URI: https://scidar.kg.ac.rs/handle/123456789/9511
Тип: Article
DOI: 10.1063/1.4791649
ISSN: 19321058
SCOPUS: 84874727126
Налази се у колекцијама:Faculty of Engineering, Kragujevac
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