The effects of sulfur-containing compounds on redox status in homocysteine-treated rats

Abstract

© 2019 Polish Pharmaceutical Society. All Rights Reserved. There is growing interest in the activity of sulfur-containing compounds on redox balance in physiological and pathological conditions, considering that some of these compounds have not only antioxidative but also pro-oxidative activities. The aim of this study was to assess possible differences in the effects of various sulfur-containing compounds on redox balance of cardiovascular system in its physiological state and in the early onset of hyperhomocysteinemia. This experimental study divided Wistar albino rats into two groups: Saline-treated (control) and DL-homocysteine-treated (experimental group). Rats from experimental group were subjected to subchronic subcutaneous administration of DL-homocysteine at dose of 0.45 Imol/g body weight twice a day for 2 weeks. At the end of this period, rats were sacrificed, and blood samples were collected to be analyzed for homocysteine concentration and systemic oxidative stress. Isolated rat hearts were excised and attached to the Langendorff apparatus. To assess the effects of acute administration of L-methionine, L-cysteine, N-acetylcysteine, and sodium hydrogen sulfide, the hearts were perfused individually with each of the mentioned substances at the same single dose of 0.5 mmol/L for 5 min. In collected samples of coronary venous effluent oxidative stress biomarkers were determined using spectrophotometry. Total homocysteine level was significantly higher in the experimental group than in the control group, and the effects of applied sulfur-containing compounds were significantly different in experimental and control groups. DL-homocysteine induced considerable changes in the functioning of a cardiovascular system even before an increase in plasma homocysteine values, and action of sulfur-containing compounds varied depending on the presence of homocysteine.