Молимо вас користите овај идентификатор за цитирање или овај линк до ове ставке: https://scidar.kg.ac.rs/handle/123456789/11083
Назив: Further insights into ruthenium(II) piano-stool complexes with N-alkyl imidazoles
Аутори: Djukic, Maja
Jeremić, Marija
Jelic, Ratomir
Klisuric, Olivera
Kojić, Vesna
Jakimov, Dimitar
Djurdjevic, Predrag
Matović, Zoran
Датум издавања: 2018
Сажетак: © 2018 Elsevier B.V. Two piano-stool ruthenium(II) complexes [Ru(η6-p-cymene)(N-MeIm)3]Cl2·2H2O (1) and [Ru(η6-p-cymene)(N-PrIm)Cl2] (2) respectively have been synthesized and characterized by elemental, spectral and structural analysis. Crystal structures of (1) and (2) have been verified by X-ray diffraction analysis. Docking experiments toward DNA dodecamer have been done. Good ΔG binding values of the complexes with imidazole derivatives comparable with ethylene-diamine complex indicate a high potential of these compounds in the formation of DNA lesions and therefore their good cytotoxic status. The interaction of CT-DNA with ruthenium(II) complexes has been studied by means of absorption and fluorescence measurements. The binding constant, Kb and the Stern–Volmer quenching constant reveal that complex (2) binds well to CT-DNA. The cytotoxic activity of Ru(II) complexes with N-RIm (R = methyl or propyl) were evaluated by MTT assay. A-549, HT-29 and HeLa cells were sensitive to all compounds tested, while the breast carcinoma cell line MCF-7 was resistant only to the complex (1). Flow cytometric analysis and fluorescent microscopy showed that ruthenium(II) complexes in HeLa cells induce apoptosis and G0/G1 cell cycle arrest and almost completely inhibit DNA synthesis. Western blot also demonstrated proteolytic cleavage of poly-(ADP-ribose) polymerase (PARP) in HeLa cells after treatment with both tested substances.
URI: https://scidar.kg.ac.rs/handle/123456789/11083
Тип: article
DOI: 10.1016/j.ica.2018.08.031
ISSN: 0020-1693
SCOPUS: 2-s2.0-85052726828
Налази се у колекцијама:Faculty of Medical Sciences, Kragujevac
Faculty of Science, Kragujevac

Број прегледа

231

Број преузимања

7

Датотеке у овој ставци:
Датотека Опис ВеличинаФормат 
PaperMissing.pdf
  Ограничен приступ
29.86 kBAdobe PDFСличица
Погледајте


Ставке на SCIDAR-у су заштићене ауторским правима, са свим правима задржаним, осим ако није другачије назначено.