The simbiotic effect of temperature and sugars on the planktonic growth and biofilm formation of Klebsiella spp. isolated from traditionally made cheese

Abstract

In this paper, we investigated the influence of temperatures (4°C, 37°C, 44°C) and different concentrations of glucose and lactose (0.5%, 1.5%, 2.5%, 3.5%) on the planktonic growth, biofilm formation and formed biofilm of Klebsiella oxytoca, Klebsiella ornithinolytica and Klebsiella pneumonia in two different broths (TSB and MH). The bacteria were isolated from the cheese produced in Southeastern Serbia. Klebsiella pneumoniae ATCC 70063 was used as a positive control. The planktonic growth and biofilm formation were measured using spectrophotometric method. Different broths caused a different planktonic growth and biofilm formation. At 4°C, the planktonic growth was not observed, neither was the formation of biofilm. Temperatures of 37°C and 44°C, as well as various concentrations of glucose and lactose, stimulated the planktonic growth of bacteria. The biofilm formation was less affected by glucose, but lactose stimulated the biofilm formation. The results of these were in accordance with the origin of bacteria, since the isolates were obtained from cheese. The intensity of the effect of sugars on the planktonic growth and biofilm formation depended on the type of bacteria.