Bioaccumulation of metals, total phenolic and flavonoid contents and antioxidant activity of Rumex acetosella L. from tailings in Žitkovac (Kosovo & Metohija)

Abstract

The aim of this study was to determine the bioaccumulation of the metals in Rumex acetosella L. collected from the tailings of abandoned mine in Žitkovac (near Kosovska Mitrovica, Kosovo and Metohija), and to establish the possibility of using those plants for medicinal purposes. Concentrations of heavy metals (Mn, Fe, Pb, Ni, Cr, Cu, Cd, Zn, Ca and Mg) in soil, roots and aboveground parts of R. acetosella were determined by wet mineralization with nitric and perchloric acid. The results of the research indicate that R. acetosella accumulates large amounts of Ni, Cu, Pb, Zn, Cr, and Mn. The total phenol content, the total amount of flavonoids and the antioxidant activity of three different extracts of the root and aboveground parts of R. acetosella were determined by spectrophotometric methods. The acetone extracts contain the highest concentration of phenols and flavonoids. Ethyl acetate has proven to be a solvent that releases the least amount of phenols and flavonoids. The methanol extract of aboveground parts has the highest, while acetone root extract has the lowest antioxidant activity. Rumex acetosella can be used for medicinal purposes, but only collected from uncontaminated soil.