DNA DAMAGE INDUCED BY SELECTED PYRAZOLINES IN RAT LIVER USING COMET ASSAY

Abstract

Pyrazolines are well known biological agents with various pharmacologically activities. As far as we know there is no previous study aimed at determining the genotoxicity of pyrazolines. The in vivo comet assay was performed to evaluate, whether or not eight selected pyrazoline derivatives with vanillic and ferrocenyl fragments, namely 3c, 3f, 4d, 4e, 5e, 5f, 6c, and 6d can damage the DNA in rat livers. No statistically significant difference was observed in the 4d, 4e, 5e, and 6d-treated groups upon comparison with the negative control. Significantly increase of the total comet score was detected in the 3c, 3f, 5f, and 6c treatment groups in comparison with the rats in the negative control group and significant less total score than those of the positive control group. According to the in vivo genotoxicity assays, propyl or butyl derivatives (4d, 4e, 5e, and 6d) seem to be the most promising substrates due to their non-genotoxic effect.