SESELI RIGIDUM WALDST. & KIT: SECONDARY METABOLITES AND ANTIOXIDANT ACTIVITY OF METHANOL, ETHYL ACETATE AND ACETONE EXTRACTS

Abstract

Representatives of the Seseli genus are recognized for their biological activities. The objective of this study was to analyze the phenolic and flavonoid content, along with the antioxidant effects, of methanol, ethyl acetate, and acetone extracts obtained from the roots, leaves, stems, and fruits of S. rigidum. Through spectrophotometric measurements of phenols and flavonoids, the results revealed that the polar (methanol) solvent extract from the leaves exhibited the highest concentrations of phenols (98.66 ± 2.64 mg GA/g) and flavonoids (20.74 ± 0.55 mg RU/g), surpassing those obtained from the stem. Specifically, the methanol extract from the leaves demonstrated the highest antioxidant activity at 25.87 ± 0.05 μg/mL, while the fruit extract exhibited the lowest antioxidant activity at 638.5 ± 0.01 μg/mL. Notably, the total phenol content displayed a strong positive correlation with the antioxidant activity of the extracts, unlike the total flavonoid content.