ANTIMICROBIAL ACTIVITY OF HYBRIDS OF COUMARIN’S DERIVATIVES WITH NEUROTRANSMITTERS 20242ND INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY (2024)ЗБОРНИЦИ

Abstract

In this study, the antimicrobial activity of five hybrids of 3-acetyl-4-hydroxy coumarin with neurotransmitters was determined: dopamine (1), tyramine (2), octopamine (3), norepinephrine (4) and methoxy-tyramine (5). Antimicrobial activity was assessed using the microdilution method incorporating resazurin, with determination of MIC/MMC concentrations. Testing involved twelve microorganisms, five of which were sourced from mine wastewater. The best activities were shown by substances (1) and (4) on Bacillus pumilus and Pantoea agglomerans, where the MIC was 31.8 μg/mL. Pantoea agglomerans showed the highest sensitivity to all tested substances, except (5). Tested substances didn't show activity on standard strains of bacteria and yeast. In general, substances act above the range of positive controls and have selective and limited activity.