The cytotoxicity of korbazol against murine cancer cell lines

Abstract

Background/aim: Korbazol is a natural product that has been shown to exert cytotoxic eff ects on leukemic cells in vitro. The cytotoxicity and biochemical eff ects induced by Korbazol were investigated in the murine cell lines 4T1, B16 and BCL1. Methods: The cytotoxic activity of the tested compound was assessed using a colorimetric MTT assay. The concentration of the superoxide anion radical (O 2.-) and the activity of superoxide dismutase (SOD) in the samples were determined using spectrophotometric methods. The MDA content was determined using a TBA assay. Results: We found that Korbazol induced cell toxicity, an increased the concentration of the lipid peroxidation end product MDA, decreased superoxide dismutase activity and increased superoxide anion formation. Conclusions: Altogether, these results suggest that oxidative stress is involved in Korbazol-induced cytotoxicity in the investigated cell lines.