Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/12515
Full metadata record
DC FieldValueLanguage
dc.rights.licenserestrictedAccess-
dc.contributor.authorSCORCIAPINO, MARIANO ANDREA-
dc.contributor.authorManzo G.-
dc.contributor.authorRinaldi, Andrea-
dc.contributor.authorSanna R.-
dc.contributor.authorCasu M.-
dc.contributor.authorPantic, Jelena-
dc.contributor.authorLukic, Miodrag-
dc.contributor.authorConlon, John Michael-
dc.date.accessioned2021-04-20T21:01:23Z-
dc.date.available2021-04-20T21:01:23Z-
dc.date.issued2013-
dc.identifier.issn0006-2960-
dc.identifier.urihttps://scidar.kg.ac.rs/handle/123456789/12515-
dc.description.abstractPlasticin-L1 (GLVNGLLSSVLGGGQGGGGLLGGIL) is a conformationally flexible glycine/leucine-rich peptide originally isolated from norepinephrine-stimulated skin secretions of the South-American Santa Fe frog Leptodactylus laticeps (Leptodactylidae). A nuclear magnetic resonance/molecular dynamics characterization of plasticin-L1 in the presence of dodecylphosphocholine (DPC) and DPC/sodium dodecylsulphate micelles as membrane-mimetic models showed that the peptide has affinity for both neutral and anionic membranes. The peptide adopts a stable helical conformation at the N-terminal region and a more disordered helix at the C-terminal region, separated by an unstructured loop wherein the highest number of glycines is localized. In both micelle environments, plasticin-L1 slowly inserts between the detergent head groups but always remains localized at the micelle/water interface. Plasticin-L1 lacks direct antimicrobial activity but stimulates cytokine production by macrophages. Incubation with plasticin-L1 (20 μg/mL) significantly (P < 0.05) increased the production of the proinflammatory cytokines IL-1β, IL-12, IL-23, and TNF-α from unstimulated peritoneal macrophages from both C57BL/6 and BALB/C mice. The peptide also increased IL-6 production by unstimulated (P < 0.01) and lipopolysaccharide-stimulated (P < 0.01) macrophages, whereas the effects on production of the anti-inflammatory cytokine IL-10 were not significant. These findings suggest that plasticin-L1 may play an immunomodulatory role in vivo by stimulating cytokine production from frog skin macrophages in response to microbial pathogens. This peptide may represent a template for the design of peptides with therapeutic applications as immunostimulatory agents. © 2013 American Chemical Society.-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourceBiochemistry-
dc.titleConformational analysis of the frog skin peptide, plasticin-L1, and its effects on production of proinflammatory cytokines by macrophages-
dc.typearticle-
dc.identifier.doi10.1021/bi4008287-
dc.identifier.scopus2-s2.0-84886057184-
Appears in Collections:Faculty of Medical Sciences, Kragujevac

Page views(s)

489

Downloads(s)

12

Files in This Item:
File Description SizeFormat 
PaperMissing.pdf
  Restricted Access
29.86 kBAdobe PDFThumbnail
View/Open


Items in SCIDAR are protected by copyright, with all rights reserved, unless otherwise indicated.