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Назив: Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model
Аутори: Kačániová, Miroslava
Terentjeva, Margarita
Galovičová L.
Ivanišová E.
Štefániková, Jana
Valkova V.
Borotova P.
Kowalczewski, Przemysław
Kunová, Simona
Felšöciová, Soňa
Tvrda E.
Žiarovská, Jana
Benda Prokeinová R.
Vukovic, Nenad
Датум издавања: 2020
Сажетак: © 2020 by the authors. The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of Citrus aurantium essential oil (CAEO). The biofilm profile of Stenotrophonomonas maltophilia and Bacillus subtilis were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of Citrus aurantium (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of S. maltophilia and B. subtilis. A differentiated branch was obtained for early growth variants of S. maltophilia for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of B. subtilis preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against Penicillium crustosum, in the vapor phase, on bread and carrot in situ.
URI: https://scidar.kg.ac.rs/handle/123456789/12763
Тип: article
DOI: 10.3390/molecules25173956
SCOPUS: 2-s2.0-85090181561
Налази се у колекцијама:Faculty of Science, Kragujevac

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