SYNTHESIS, CHARACTERIZATION AND HSA/DNA INTERACTIONS OF NEW [RH2(CH3COO)4L2)] COMPLEX

Abstract

In this report, we have synthesized a complex of [Rh2(CH3COO)4(H2O)2] and 1-butylimidazole ligand (L). To a solution of [Rh2(CH3COO)4(H2O)2] (0.0478 g, 0.10 mmol) in toluene (10 mL) the 1-butylimidazole ligand (L) (0.0290 mL, 0.22 mmol) was added. The resulting mixture was stirred at room temperature for 1 hour until the reagents were completely dissolved. The solution was left at room temperature to evaporate slowly to a volume of 6 to 7 mL and then stored overnight in the refrigerator. The precipitated purple crystals were filtered off under vacuum and washed with diethyl ether. Characterization of the synthesized complex [Rh2(CH3COO)4L2)] was carried out by elemental microanalysis, IR, NMR and by determination of the melting point. The interactions of the new complex with molecules of human serum albumin (HSA) and calf thymus DNA (CTDNA) were studied by fluorescence spectroscopy as well as by docking experiments on the mentioned molecules. The high value of the binding constant, Kb, and the Stern-Volmer quenching constant, KSV, are the result of good binding of the complex to HSA and CT-DNA.