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Title: Antiproliferative effect of 13-cis-retinoic acid is associated with granulocyte differentiation and decrease in cyclin B1 and BCL-2 protein levels in G0/G1 arrested HL-60 cells
Authors: Vuletić A.
Konjević G.
Milanović D.
Ruždijić S.
Jurisic, Vladimir
Issue Date: 2010
Abstract: Retinoic acid (RA), similar to specific growth factors, can induce differentiation of proliferating promyelocytic precursors into terminally differentiated granulocytes, although little is known about effects of its 13-cis isomer on promyelocytic leukemia (PML). In this study we demonstrate that 13-cis-RA has a dose and time-dependant antiproliferative effect on HL-60 PML cell line, that it induces cell accumulation in resting G0/G1 phase of the cell cycle followed by an increase in CD11b granulocyte differentiation antigen expression. The obtained increase in the percentage of HL-60 cells in G0/G1 phase and complementary decrease in S phase of the cell cycle are accompanied by a decrease in the expression of cell cycle regulatory molecule cyclin B1. We also show the induction of interferon regulatory factor-1 (IRF-1) transcription that can, also, to some extent contribute to the antiproliferative effect of 13-cis-RA. Furthermore, down-regulation of Bcl-2 protein expression in 13-cis-RA treated HL-60 cells may contribute to sensitivity to apoptosis of growth arrested HL-60 promyelocytic cells. © 2010 Arányi Lajos Foundation.
Type: article
DOI: 10.1007/s12253-009-9241-2
ISSN: 1219-4956
SCOPUS: 2-s2.0-77956886917
Appears in Collections:Faculty of Medical Sciences, Kragujevac

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