Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/11557
Title: Comparison of the Rhodotorula mucilaginosa Biofilm and Planktonic Culture on Heavy Metal Susceptibility and Removal Potential
Authors: Grujić N.
Vasić, Sava
Radojevic, Ivana
Čomić, Ljiljana
Ostojić, Aleksandar
Journal: Water, Air, and Soil Pollution
Issue Date: 1-Feb-2017
Abstract: © 2017, Springer International Publishing Switzerland. This study compares the effect of heavy metals (Hg2+, Cu2+, and Pb2+) on the Rhodotorula mucilaginosa and Saccharomyces boulardii biofilm and planktonic cells. A MBECTM-HTP assay was used to test the levels of tolerance to heavy metals. The minimum inhibitory concentration (MICp) and minimum lethal concentration (MLCp) of the R. mucilaginosa and S. boulardii planktonic cells were determined, as well as minimum biofilm eradication concentration (MBEC). Metal removal efficiency was determined by batch biosorption assay. Previous studies had focused on heavy metal tolerance and removal efficiency of planktonic cells from Rhodotorula species only. Hence, our study presents and compares results for metal tolerance and removal efficiency of the R. mucilaginosa planktonic cells and biofilm. Biofilm tolerance was higher than the planktonic cells. The R. mucilaginosa planktonic cells showed the tolerance in the presence of Hg2+(MICp 0.08 mM), Cu2+(MICp 6.40 mM), and Pb2+(MICp 3.51 mM), while the S. boulardii planktonic cells only tolerated Pb2+(MICp 0.43 mM). The R. mucilaginosa biofilm showed the highest tolerance in the presence of Hg2+(MBEC >0.31 mM), Cu2+(MBEC >12.81 mM), Pb2+(MBEC >7.12 mM), and obtained results were confirmed by fluorescence microscopy. S. boulardii did not show potential in biofilm formation. The R. mucilaginosa biofilm exhibited better efficiency in removal of all tested metals than the planktonic cells. Metal removal efficiency was in the range from 4.79–10.25% for planktonic cells and 91.71–95.39% for biofilm.
URI: https://scidar.kg.ac.rs/handle/123456789/11557
Type: article
DOI: 10.1007/s11270-017-3259-y
ISSN: 00496979
SCOPUS: 85010818146
Appears in Collections:Faculty of Science, Kragujevac

Page views(s)

60

Downloads(s)

1

Files in This Item:
File Description SizeFormat 
PaperMissing.pdf
  Restricted Access
29.86 kBAdobe PDFThumbnail
View/Open


Items in SCIDAR are protected by copyright, with all rights reserved, unless otherwise indicated.