Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/11647
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dc.contributor.authorKatanić Stanković, Jelena S.-
dc.contributor.authorJurić, Tatjana-
dc.contributor.authorMihailovic, Vladimir-
dc.contributor.authorNIKLES S.-
dc.contributor.authorpan, sudip-
dc.contributor.authorRosic, Gvozden-
dc.contributor.authorSelakovic, Dragica-
dc.contributor.authorJoksimovic, Jovana-
dc.contributor.authorMitrović I.-
dc.contributor.authorBauer, Rudolf-
dc.date.accessioned2021-04-20T18:52:50Z-
dc.date.available2021-04-20T18:52:50Z-
dc.date.issued2016-
dc.identifier.issn0378-8741-
dc.identifier.urihttps://scidar.kg.ac.rs/handle/123456789/11647-
dc.description.abstract© 2016 Elsevier Ireland Ltd Ethnopharmacological relevance Meadowsweet (Filipendula ulmaria (L.) Maxim, Rosaceae) has been traditionally used in most European countries for the treatment of inflammatory diseases due to its antipyretic, analgesic, astringent, and anti-rheumatic properties. However, there is little scientific evidence on F. ulmaria anti-inflammatory effects regarding its impact on cyclooxygenases enzymatic activity and in vivo assessment of anti-inflammatory potential. This study aims to reveal the anti-inflammatory activity of methanolic extracts from the aerial parts (FUA) and roots (FUR) of F. ulmaria, both in in vitro and in vivo conditions. Materials and methods The characteristic phenolic compounds in F. ulmaria extracts were monitored via high performance thin layer chromatography (HPTLC). The in vitro anti-inflammatory activity of F. ulmaria extracts was evaluated using cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) enzyme assays, and an assay for determining COX-2 gene expression. The in vivo anti-inflammatory effect of F. ulmaria extracts was determined in two doses (100 and 200 mg/kg b.w.) with hot plate test and carrageenan-induced paw edema test in rats. Inflammation was also evaluated by histopathological and immunohistochemical analysis. Results FUA extract showed the presence of rutoside, spiraeoside, and isoquercitrin. Both F. ulmaria extracts at a concentration of 50 μg/mL were able to inhibit COX-1 and -2 enzyme activities, whereby FUA extract (62.84% and 46.43% inhibition, respectively) was double as effective as the root extract (32.11% and 20.20%, respectively). Extracts hardly inhibited the level of COX-2 gene expression in THP-1 cells at a concentration of 25 μg/mL (10.19% inhibition by FUA and 8.54% by FUR). In the hot plate test, both extracts in two doses (100 and 200 mg/kg b.w.), exhibited an increase in latency time when compared with the control group (p<0.05). In the carrageenan-induced acute inflammation test, FUA at doses of 100 and 200 mg/kg b.w., and FUR at 200 mg/kg, were able to significantly reduce the mean maximal swelling of rat paw until 6 h of treatment. Indomethacin, FUA, and FUR extracts significantly decreased inflammation score and this effect was more pronounced after 24 h, compared to the control group (p<0.05). Conclusions The observed results of in vitro and, for the first time, in vivo anti-inflammatory activity of meadowsweet extracts, provide support of the traditional use of this plant in the treatment of different inflammatory conditions. Further investigation of the anti-inflammatory compounds could reveal the mechanism of anti-inflammatory action of these extracts.-
dc.rightsrestrictedAccess-
dc.sourceJournal of Ethnopharmacology-
dc.titleIn vitro and in vivo assessment of meadowsweet (Filipendula ulmaria) as anti-inflammatory agent-
dc.typearticle-
dc.identifier.doi10.1016/j.jep.2016.10.015-
dc.identifier.scopus2-s2.0-84994226043-
Appears in Collections:Faculty of Medical Sciences, Kragujevac
Faculty of Science, Kragujevac
Institute for Information Technologies, Kragujevac

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