Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/11697
Title: Purification, Conformational Analysis, and Properties of a Family of Tigerinin Peptides from Skin Secretions of the Crowned Bullfrog Hoplobatrachus occipitalis
Authors: McLaughlin C.
Lampis S.
Mechkarska, Milena
COQUET, Laurent
jouenne, thierry
King J.
L. Mangoni M.
Lukic, Miodrag
SCORCIAPINO, MARIANO ANDREA
Conlon, John Michael
Issue Date: 2016
Abstract: © 2016 The American Chemical Society and American Society of Pharmacognosy. Four host-defense peptides belonging to the tigerinin family (tigerinin-1O: RICTPIPFPMCY; tigerinin-2O: RTCIPIPLVMC; tigerinin-3O: RICTAIPLPMCL; and tigerinin-4O: RTCIPIPPVCF) were isolated from skin secretions of the African crowned bullfrog Hoplobatrachus occipitalis. In aqueous solution at pH 4.8, the cyclic domain of tigerinin-2O adopts a rigid amphipathic conformation that incorporates a flexible N-terminal tail. The tigerinins lacked antimicrobial (MIC > 100 μM) and hemolytic (LC50 > 500 μM) activities but, at a concentration of 20 μg/mL, significantly (P < 0.05) inhibited production of interferon-γ (IFN-γ) by peritoneal cells from C57BL/6 mice without affecting production of IL-10 and IL-17. Tigerinin-2O and -4O inhibited IFN-γ production at concentrations as low as 1 μg/mL. The tigerinins significantly (P ≤ 0.05) stimulated the rate of insulin release from BRIN-BD11 clonal β-cells without compromising the integrity of the plasma membrane. Tigerinin-1O was the most potent (threshold concentration 1 nM) and the most effective (395% increase over basal rate at a concentration of 1 μM). Tigerinin-4O was the most potent and effective peptide in stimulating the rate of glucagon-like peptide-1 release from GLUTag enteroendocrine cells (threshold concentration 10 nM; 289% increase over basal rate at 1 μM). Tigerinin peptides have potential for development into agents for the treatment of patients with type 2 diabetes.
URI: https://scidar.kg.ac.rs/handle/123456789/11697
Type: article
DOI: 10.1021/acs.jnatprod.6b00494
ISSN: 0163-3864
SCOPUS: 2-s2.0-84988837626
Appears in Collections:Faculty of Medical Sciences, Kragujevac

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