Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/12144
Title: ST2 deletion increases inflammatory bone destruction in experimentally induced periapical lesions in mice
Authors: Velickovic M.
Pejnović, Nada
Mitrović P.
Radosavljevic, Gordana
Jovanovic I.
Kanjevac, Tatjana
Jovicic, Nemanja
Lukic, Aleksandra
Journal: Journal of Endodontics
Issue Date: 1-Jan-2015
Abstract: © 2015 American Association of Endodontists. Introduction ST2 is a member of the interleukin (IL)-1 receptor family, and IL-33 is its natural ligand. ST2 signaling promotes Th2 immune response in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. The purpose of this study was to investigate whether ST2 gene deletion affects the development of experimentally induced periapical lesions in mice. Methods Pulps of mandibular molars from wild-type (WT) and ST2 knockout (ST2-/-) BALB/c mice were exposed and left open to the oral environment. After death, hemi-mandibles were isolated and prepared for histologic, immunohistochemical, and flow cytometric analysis. Results The expression of IL-33 and its receptor ST2 was higher in periapical lesions in WT mice compared with normal root apices (both P <.05). The increased periapical bone loss observed in ST2-/- mice was associated with enhanced influx of neutrophils, CD3+ CXCR3+ Th1 cells, and CD3+ CCR6+ Th17 cells and increased number of tartrate-resistant acid phosphatase+ osteoclasts (all P <.05). Furthermore, periapical lesions in ST2-/- mice contained increased percentages of T cells expressing interferon-γ, IL-17, tumor necrosis factor-α, and IL-6 (all P <.05). In comparison with WT mice, CD3+ receptor activator of nuclear factor kappa B ligand+ T cells were increased, whereas CD3+ osteoprotegerin+ T cells were decreased in the lesions of ST2-/- mice (both P <.05). Conclusions ST2 deletion increases inflammatory bone loss in experimental periapical lesions in mice, which is associated with enhanced Th1/Th17 cell mediated periapical immune responses and increased osteoclastogenesis.
URI: https://scidar.kg.ac.rs/handle/123456789/12144
Type: article
DOI: 10.1016/j.joen.2014.11.017
ISSN: 00992399
SCOPUS: 84924258468
Appears in Collections:Faculty of Medical Sciences, Kragujevac

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