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Title: The silk of Plodia interpunctella as a potential biomaterial and its cytotoxic effect on cancer cells
Authors: Ćurčić Milutinović, Milena
Čurović D.
Nikodijević, Danijela
Vukajlović, Filip
Predojević, Dragana
Marković, Snežana
Pesic, Snezana
Issue Date: 2020
Abstract: © 2019, © 2019 Taylor & Francis Group, LLC. Insect silk has been widely studied for its application in regenerative medicine. However, the data about Plodia interpunctella silk as a biomaterial and its anticancer properties are insufficient. Thus, the aim of this study was to investigate native silk as a substrate for growing normal human fibroblasts MRC-5, and test potential cytotoxic effects of the two silk extracts (with DMSO and Trypsin for sericin isolation) on HCT-116 colorectal carcinoma cells and MRC-5 fibroblasts as a control. Fifth-instar larval silk, collected for 15 and 30 days, was used for testing of proliferation and adhesion of MRC-5, 24 h and 72 h after seeding. Light- and fluorescence-microscope showed cell adhesion and spread on silk, as well as enhanced number of cells after 72 compared to 24 h and nonsignificant percentage of apoptotic cells on the silk. Although insoluble, P. interpunctella silk showed remarkable cytotoxic activity on HCT-116 cells, without significant cytotoxity on normal fibroblasts after 24 h and weak effects after 72 h. This study provides significant information about P. interpunctella silk as a potential biomaterial and shows the presence of some active constituents with anticancer properties, thus pointing to the possibility for exploitation of this worldwide pest insect in biomedical application.
Type: article
DOI: 10.1080/10495398.2019.1575848
ISSN: 1049-5398
SCOPUS: 2-s2.0-85062358182
Appears in Collections:Faculty of Science, Kragujevac

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