Please use this identifier to cite or link to this item:
https://scidar.kg.ac.rs/handle/123456789/12844
Title: | The silk of Plodia interpunctella as a potential biomaterial and its cytotoxic effect on cancer cells |
Authors: | Ćurčić Milutinović, Milena ![]() ![]() Čurović D. Nikodijević, Danijela ![]() ![]() Vukajlović, Filip ![]() ![]() Predojević, Dragana ![]() ![]() Marković, Snežana ![]() ![]() Pesic, Snezana ![]() ![]() |
Issue Date: | 2020 |
Abstract: | © 2019, © 2019 Taylor & Francis Group, LLC. Insect silk has been widely studied for its application in regenerative medicine. However, the data about Plodia interpunctella silk as a biomaterial and its anticancer properties are insufficient. Thus, the aim of this study was to investigate native silk as a substrate for growing normal human fibroblasts MRC-5, and test potential cytotoxic effects of the two silk extracts (with DMSO and Trypsin for sericin isolation) on HCT-116 colorectal carcinoma cells and MRC-5 fibroblasts as a control. Fifth-instar larval silk, collected for 15 and 30 days, was used for testing of proliferation and adhesion of MRC-5, 24 h and 72 h after seeding. Light- and fluorescence-microscope showed cell adhesion and spread on silk, as well as enhanced number of cells after 72 compared to 24 h and nonsignificant percentage of apoptotic cells on the silk. Although insoluble, P. interpunctella silk showed remarkable cytotoxic activity on HCT-116 cells, without significant cytotoxity on normal fibroblasts after 24 h and weak effects after 72 h. This study provides significant information about P. interpunctella silk as a potential biomaterial and shows the presence of some active constituents with anticancer properties, thus pointing to the possibility for exploitation of this worldwide pest insect in biomedical application. |
URI: | https://scidar.kg.ac.rs/handle/123456789/12844 |
Type: | article |
DOI: | 10.1080/10495398.2019.1575848 |
ISSN: | 1049-5398 |
SCOPUS: | 2-s2.0-85062358182 |
Appears in Collections: | Faculty of Science, Kragujevac |
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File | Description | Size | Format | |
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Milutinovic et al 2019 Plodia.pdf Restricted Access | 1.18 MB | Adobe PDF | View/Open |
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