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Назив: 3D HeLa spheroids as a model for investigating the anticancer activity of Biginelli-hybrids
Аутори: Djordjic Crnogorac, Marija
Matic, Ivana
Damjanovic, Ana
Janković, Nenad
Krivokuca, Ana
Stanojkovic, Tatjana
Часопис: Chemico-Biological Interactions
Датум издавања: 25-авг-2021
Сажетак: In previous study, we examined the anticancer effects of novel Biginelli-hybrids against HeLa cell line on 2D monolayer culture. The five most effective compounds were chosen for further analysis of their anticancer activity against HeLa spheroids. Using the 3D models implies the possible differences in anticancer effects and mechanisms of activity of tested compounds. The compounds 4c and 4d exerted the strongest activity against 3D HeLa spheroids and induced to some extent loosened cell-cell contacts in spheroids, leading to the largest reduction in the diameter of the spheroids. Additionally, the highest accumulation of the cells in the subG1 phase of the cell cycle was observed after the treatment with compounds 4d and 4c, while the compound 4f led to the G2/M arrest. The invasion potential of treated HeLa cells in spheroids was monitored by imaging of spheroids embedded in a matrix made of matrigel and collagen and by determination of MMP2, MMP9, and VEGF gene expression levels. The compound 4l did not show invasion-suppressive activity, while the compounds 4c and 4d exerted the strongest anti-invasive activity.
URI: https://scidar.kg.ac.rs/handle/123456789/13605
Тип: journal article
DOI: 10.1016/j.cbi.2021.109565
ISSN: 00092797
SCOPUS: 85108443615
Налази се у колекцијама:Institute for Information Technologies, Kragujevac

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