Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/13800
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dc.rights.licenseopenAccess-
dc.contributor.authorRadisavljević, Snežana-
dc.contributor.authorĐeković Kesić, Ana-
dc.contributor.authorĆoćić, Dušan-
dc.contributor.authorPuchta, Ralph-
dc.contributor.authorSenft, Laura-
dc.contributor.authorĆurčić Milutinović, Milena-
dc.contributor.authorMilivojević, Nevena-
dc.contributor.authorPetrović, Biljana-
dc.date.accessioned2021-12-14T11:30:28Z-
dc.date.available2021-12-14T11:30:28Z-
dc.date.issued2020-
dc.identifier.issn1144-0546en_US
dc.identifier.urihttps://scidar.kg.ac.rs/handle/123456789/13800-
dc.description.abstractTwo gold(III) complexes, square-planar [Au(DPP)Cl2]+(1) and distorted square-pyramidal [Au(DMP)Cl3] (2) (where DMP = 2,9-dimethyl-1,10-phenanthroline and DPP = 4,7-diphenyl-1,10-phenanthroline), were studied by different experimental methods. Their stability in water and in buffer solution (25 mM Hepes, 30 mM NaCl, pH = 7.2) was investigated by UV-Vis spectroscopy while their redox stability is confirmed by CV. Substitution reactions between complexes 1 and 2, and biologically relevant ligands, such as thiourea (Tu), guanosine-5′-monophosphate (5′-GMP), glutathione (GSH) and L-methionine (L-Met), were studied by a stopped-flow technique, under the pseudo-first order conditions as a function of ligand concentration and temperature. According to the values of the activation parameters, all studied reactions followed an associative substitution mechanism. DNA binding studies of complexes 1 and 2 were performed by UV-Vis and fluorescence spectroscopy and viscosity measurements, as well as interactions with bovine serum albumin (BSA). Density functional theory (DFT) was implemented in order to analyse the wave function of the optimized structures to get better insight into the binding interactions between the inert ligands and gold(III) center. The experimental results of binding studies with DNA and BSA were simulated and compared by performing a molecular docking study. All results demonstrate the strong connection between the reactivity of the complexes toward biologically important targets and their structural and electronic characteristics. The cytotoxic activity of complexes 1 and 2 against different cell lines (MDA-MB-231, HCT-116, and HaCaT) was evaluated 24 and 72 h after treatments. The results indicate reduced viability of cell lines in a time- and dose-dependent manner.en_US
dc.publisherNew Journal of Chemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.sourceNew Journal of Chemistryen_US
dc.subjectgold(III)en_US
dc.subjectDNAen_US
dc.subjectBSAen_US
dc.subjectsubstitutionen_US
dc.subjectcytotoxicityen_US
dc.titleStudies of the stability, nucleophilic substitution reactions, DNA/BSA interactions, cytotoxic activity, DFT and molecular docking of some tetra- and penta-coordinated gold(iii) complexesen_US
dc.typearticleen_US
dc.description.versionAccepted for publishingen_US
dc.identifier.doi10.1039/D0NJ02037Ken_US
dc.type.versionReviewedVersionen_US
Appears in Collections:Faculty of Science, Kragujevac
Institute for Information Technologies, Kragujevac

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