Coupling Experimental Data and Numerical Simulation in Modelling Epithelial Barrier Formation with A549 Cancerous Cell Line

Abstract

The overall aim of organ-on-a-chip monitoring model tissues is providing self-reporting tissue models that can either individually or as an integrated platform be used to test the biocompatibility of materials with some emphasis on materials immune-compatibility/immune-toxicity. We will focus in this abstract on coupling the experimental data with numerical simulations for the purposes of epithelial barrier formation with A549 cancerous cell line. The collected data included pictures,which have been acquired using bright field microscope at different time points: 30min,4h,24h,48h and 72h,for calculation of the spreading and proliferation coefficient of A549 cell line. Developed model will provide insight into cells formation of the epithelial barrier. Calculated input parameters – characteristics of the chosen cell line: spreading and proliferation coefficients,seem appropriate and in accordance to the experimental outputs. Cells growth,migration and proliferation are modelled with a sort of extended diffusion equation. The preferred result of the simulation is time needed for formation of the barrier. This model will further become more complex in the view of getting desired outputs.