Qualitative content of selected phenolic compounds in different extracts of plant species Iris pumila L

Abstract

The aim of this paper is to determine the content of selected phenolic compounds and to find the appropriate optimal extraction agent for the extraction of phenolic compounds from the plant species Iris pumila L. The content of total phenols and flavonoids in the rhizome and aboveground parts of the plant was determined by the application of the spectrophotometric method in the examined extracts obtained by the maceration method. By comparatively analyzing the application of two solvents for extraction, we concluded that the application of methanol in the conventional maceration technique contributes to a higher yield of phenolic compounds. Using methanol as a solvent, it was found that the content of selected phenolic compounds in the rhizome (28.57 mg/g extract total phenolic and for total flavonoids 24.21 mg/g extract) compared to ethyl acetate as solvent. Tested extracts of parts of Iris pumila L. plant contain high levels of phenolic compounds and are suitable for use as a source of compounds with pronounced antioxidant activity.