Evaluation of the anticancer and antibiofilm properties of Agrimonia eupatoria L. silver nanoparticles

Abstract

This study evaluates the cytotoxic and antibiofilm effects of silver nanoparticles (AgNPs) synthesised with aqueous and acetone extracts of Agrimonia eupatoria L. AgNPs significantly reduced HCT-116 colorectal cancer cell viability after 72 h, with AgNPs-acetone showing stronger effects but AgNPs-H₂O being more effective at lower doses. AgNPs-H₂O also inhibited biofilm formation by clinically relevant bacteria, with a wastewater isolate of Pseudomonas aeruginosa being the most sensitive. The results confirm the strain- and mediumspecific activity of these AgNPs and support their potential as multifunctional anticancer and antibiofilm agents. Further studies are needed to assess their selectivity, safety, and mechanisms of action.