Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/8327
Title: SDF-1/CXCR4 signalling is involved in blood vessel growth and remodelling by intussusception
Authors: Dimova I.
Karthik S.
Makanya, Andrew
Hlushchuk R.
Semela D.
Volarevic, Vladislav
Djonov V.
Journal: Journal of Cellular and Molecular Medicine
Issue Date: 1-Jun-2019
Abstract: © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. The precise mechanisms of SDF-1 (CXCL12) in angiogenesis are not fully elucidated. Recently, we showed that Notch inhibition induces extensive intussusceptive angiogenesis by recruitment of mononuclear cells and it was associated with increased levels of SDF-1 and CXCR4. In the current study, we demonstrated SDF-1 expression in liver sinusoidal vessels of Notch1 knockout mice with regenerative hyperplasia by means of intussusception, but we did not detect any SDF-1 expression in wild-type mice with normal liver vessel structure. In addition, pharmacological inhibition of SDF-1/CXCR4 signalling by AMD3100 perturbs intussusceptive vascular growth and abolishes mononuclear cell recruitment in the chicken area vasculosa. In contrast, treatment with recombinant SDF-1 protein increased microvascular density by 34% through augmentation of pillar number compared to controls. The number of extravasating mononuclear cells was four times higher after SDF-1 application and two times less after blocking this pathway. Bone marrow-derived mononuclear cells (BMDC) were recruited to vessels in response to elevated expression of SDF-1 in endothelial cells. They participated in formation and stabilization of pillars. The current study is the first report to implicate SDF-1/CXCR4 signalling in intussusceptive angiogenesis and further highlights the stabilizing role of BMDC in the formation of pillars during vascular remodelling.
URI: https://scidar.kg.ac.rs/handle/123456789/8327
Type: Article
DOI: 10.1111/jcmm.14269
ISSN: 15821838
SCOPUS: 85066137089
Appears in Collections:Faculty of Medical Sciences, Kragujevac
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