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Title: Ethyl acetate extracts of two Artemisia species: Analyses of phenolic profile and anticancer activities against SW-480 colon cancer cells
Authors: Jakovljevic, Mihajlo
Grujičić, Darko
Živanovic M.
Stankovíc M.
Ciric, Andrija
Djurdjevic, Predrag
Todorovic Z.
Zivancevic-Simonovic, Snezana
Mihaljević O.
Milošević-Đorđević, Olivera
Journal: Natural Product Communications
Issue Date: 1-May-2019
Abstract: © The Author(s) 2019 Article reuse guidelines: Because Artemisia vulgaris L. and Artemisia alba Turra are traditional medicinal plants used for the treatment of different diseases, we evaluated the cytotoxic/apoptotic activity of ethyl acetate extracts from these natural products against human colon cancer cells SW-480. The extracts contained a large amount of the total polyphenols and flavonoids. The phenolic profile showed the presence of phenolic acids (gallic, p-coumaric, vanillic, and ferulic acids) and flavonoids (rutin, myricetin, luteolin, quercetin, and apigenin). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay indicated that antiproliferative activities of both A. vulgaris and A. alba extracts increased with the extension of time exposure, with decreasing IC50 values. Mitomycin C (MMC) alone had antiproliferative activity, but in combination with plant extracts caused stronger effect with lower IC50 values. Flow cytometry analyses showed that A. alba extract induced higher percentage of SW-480 cells in the early stage of apoptosis (33.5 ± 1.6 vs 0.7 ± 0.1, P < 0.05), whereas the A. vulgaris extract significantly increased the percentage of cells in necrosis (82.4 ± 5.0 vs 53.9 ± 2.3, P < 0.05). In conclusion, A. alba extract can be considered a potential source of bioactive components with anticancer activity or be used as a dietary food supplement or supplement to chemotherapy due to its synergistic effect with the MMC.
Type: Article
DOI: 10.1177/1934578X19843011
ISSN: 1934578X
SCOPUS: 85066414666
Appears in Collections:Faculty of Medical Sciences, Kragujevac
Faculty of Science, Kragujevac
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