Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/8698
Title: Indoleamine 2,3-dioxygenase-dependent expansion of T-regulatory cells maintains mucosal healing in ulcerative colitis
Authors: Acovic A.
Simovic Markovic, Bojana
Gazdic, Marina
Arsenijevic A.
Jovicic, Nemanja
Gajovic N.
Jovanovic M.
Zdravković N.
Kanjevac, Tatjana
Harrell C.
Fellabaum, Crissy
Dolicanin Z.
Djonov V.
Arsenijevic, Nebojsa
Lukic, Miodrag
Volarevic, Vladislav
Journal: Therapeutic Advances in Gastroenterology
Issue Date: 1-Jan-2018
Abstract: © The Author(s), 2018. Background: Dendritic cell (DC)-derived indolamine 2,3-dioxygenase (IDO) degrades tryptophan to kynurenine, which promotes conversion of inflammatory T cells in immunosuppressive regulatory T cells (Tregs). We analyzed the significance of the IDO:Treg axis for inducing and maintaining mucosal healing in ulcerative colitis (UC). Methods: Dextran sodium sulphate (DSS)-induced colitis in BALB/c mice (model for mucosal healing) and C57BL/6 mice (model for persistent disease) was used. Serum, fecal samples and colon-infiltrating immune cells of 65 patients with UC with mucosal healing or persistent colitis were analyzed. Results: Significantly higher serum levels of kynurenine and downregulated inflammatory cytokines were noticed in DSS-treated BALB/c mice compared with C57BL/6 mice. Increased IDO activity and attenuated capacity for antigen presentation and production of inflammatory cytokines, observed in BALB/c DCs, was followed by a significantly lower number of inflammatory T helper 1 (Th1) and Th17 cells and a notably increased number of Tregs in the colons of DSS-treated BALB/c mice. DCs and Tregs were crucially important for the maintenance of mucosal healing since their depletion aggravated colitis. Mucosal healing, followed by an increase in kynurenine and intestinal Tregs, was re-established when BALB/c DCs were transferred into DC-depleted or Treg-depleted DSS-treated BALB/c mice. This phenomenon was completely abrogated by the IDO inhibitor. Significantly higher serum and fecal levels of kynurenine, accompanied by an increased presence of intestinal Tregs, were noticed in patients with UC with mucosal healing and negatively correlated with disease severity, fecal calprotectin, colon-infiltrating interferon γ and interleukin-17-producing cells, serum and fecal levels of inflammatory cytokines. Conclusion: IDO-dependent expansion of endogenous Tregs should be further explored as a new approach for the induction and maintenance of mucosal healing in patients with UC.
URI: https://scidar.kg.ac.rs/handle/123456789/8698
Type: Article
DOI: 10.1177/1756284818793558
ISSN: 1756283X
SCOPUS: 85054785771
Appears in Collections:Faculty of Medical Sciences, Kragujevac
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