Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/8749
Title: Bacterial flora play important roles in acute dextran sulphate sodium-induced colitis but are not involved in Gal-3 dependent modulation of colon inflammation
Authors: Markovic B.
Milosavljevic N.
Arsenijevic A.
Gazdic, Marina
Lukic, Miodrag
Volarevic, Vladislav
Journal: Serbian Journal of Experimental and Clinical Research
Issue Date: 1-Sep-2017
Abstract: © 2017, University of Kragujevac, Faculty of Science. All rights reserved. An altered immune response to normal gut microflora is important for the pathogenesis of ulcerative colitis (UC). Galectin- 3 (Gal-3) is an endogenous lectin that plays an important pro-inflammatory role in the induction phase of acute colitis by promoting activation of the NLRP3 inflammasome and production of IL-1β in macrophages. By using dextran sulphate sodium (DSS) induced colitis, a well-established animal model of UC, we determined whether Gal-3 affects the function of colon infiltrating macrophages by interfering with intestinal microflora. Our results showed that genetic deletion of Gal-3 significantly attenuates DSS-induced colitis by down-regulating infiltration of phagocytic cells (neutrophils, macrophages and dendritic cells) in colon tissue of DSS-treated mice, and this correlated with differences in bacterial flora of the gut. Antibiotic treatment attenuates DSS-induced colitis in WT and Gal-3-/- mice without affecting differences between the groups. In conclusion, Gram negative bacterial flora play an important role in DSS-induced acute colitis of mice but are not involved in Gal-3 dependent modulation of colon inflammation.
URI: https://scidar.kg.ac.rs/handle/123456789/8749
Type: Article
DOI: 10.1515/SJECR-2017-0022
ISSN: 18208665
SCOPUS: 85030680699
Appears in Collections:Faculty of Medical Sciences, Kragujevac
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