Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/8903
Title: Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative/antioxidative status
Authors: Matić, Miloš
Paunović, Milica
Ognjanovic, Branka
Štajn A.
Saicic Z.
Journal: Turkish Journal of Biology
Issue Date: 1-Jan-2017
Abstract: © TÜBİTAK. Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2•-, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
URI: https://scidar.kg.ac.rs/handle/123456789/8903
Type: Article
DOI: 10.3906/biy-1606-73
ISSN: 13000152
SCOPUS: 85018810634
Appears in Collections:Faculty of Science, Kragujevac
[ Google Scholar ]

Page views(s)

48

Downloads(s)

4

Files in This Item:
File Description SizeFormat 
10.3906-biy-1606-73.pdf289.82 kBAdobe PDFThumbnail
View/Open


Items in SCIDAR are protected by copyright, with all rights reserved, unless otherwise indicated.