Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/9010
Title: Galectin-3 deletion enhances visceral adipose tissue inflammation and dysregulates glucose metabolism in mice on a high-fat diet
Authors: Jeftic, Ilija
Miletic Kovacevic, Marina
Jovicic, Nemanja
Pantic, Jelena
Arsenijevic, Nebojsa
Lukic, Miodrag
Pejnović, Nada
Journal: Serbian Journal of Experimental and Clinical Research
Issue Date: 1-Sep-2016
Abstract: © 2016, University of Kragujevac, Faculty of Science. All rights reserved. Obesity and type 2 diabetes mellitus (T2DM) constitute major health problems worldwide. Increased visceral adiposity enhances the risk of insulin resistance and type 2 diabetes. The mechanisms involved in obesity-associated chronic inflammation in metabolic tissues (metaflammation) that lead to insulin resistance and dysregulated glucose metabolism are incompletely defined. Galectin-3 (Gal-3), a β-galactosidebinding lectin, modulates immune/inflammatory responses and specifically binds to metabolic danger molecules. To dissect the role of Gal-3 in obesity and diabetes, Gal-3-deficient (LGALS3-/-) and wild-type (WT) C57Bl/6 male mice were placed on a high-fat diet (HFD, 60% kcal fat) or a standard chow diet (10% kcal fat) for 6 months and metabolic, histological and immunophenotypical analyses of the visceral adipose tissue were performed. HFD-fed LGALS3-/- mice had higher body weights and more body weight gain, visceral adipose tissue (VAT), hyperglycaemia, hyperinsulinemia, insulin resistance and hyperlipidemia than diet-matched WT mice. Compared to WT mice, the enlarged VAT in obese LGALS3-/- mice contained larger adipocytes. Additionally, we demonstrate enhanced inflammation in the VAT of LGALS3-/- mice compared with diet-matched WT mice. The VAT of LGALS3-/- mice fed a HFD contained more numerous dendritic cells and proinflammatory F4/80+CD11c+CD11b+ and F4/80high macrophages. In contrast to WT mice, the numbers of CXCR3+ and CD8+ T cells were increased in the VAT of Gal-3-deficient mice after 6 months of high-fat feeding. We provide evidence that Gal-3 ablation results in enhanced HFD-induced adiposity, inflammation in the adipose tissue, insulin resistance and hyperglycaemia. Thus, Gal-3 represents an important regulator of obesity-associated immunometabolic alterations.
URI: https://scidar.kg.ac.rs/handle/123456789/9010
Type: Article
DOI: 10.1515/SJECR-2016-0030
ISSN: 18208665
SCOPUS: 84988813610
Appears in Collections:Faculty of Medical Sciences, Kragujevac
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