Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/9636
Title: Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropinom
Authors: Milosević V.
Todorovic, Danijela
Velickovic M.
Ristic, Natasa
Uš́ebrka G.
Kněević V.
Ajďanović V.
Issue Date: 2012
Abstract: Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically labeled ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG.
URI: https://scidar.kg.ac.rs/handle/123456789/9636
Type: article
DOI: 10.2478/v10011-011-0041-3
ISSN: 1452-8258
SCOPUS: 2-s2.0-80955122789
Appears in Collections:Faculty of Medical Sciences, Kragujevac

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