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https://scidar.kg.ac.rs/handle/123456789/9667
Title: | Teucrium plant species as natural sources of novel anticancer compounds: Antiproliferative, proapoptotic and antioxidant properties |
Authors: | Stankovic, Milan Ćurčić Milutinović, Milena Žižić J. Topuzovic, Marina Solujic A. Marković, Snežana |
Issue Date: | 2011 |
Abstract: | This study deals with total phenolic content, antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and the effect on the prooxidant/antioxidant status in HCT-116 cells. The total phenolic content of the extracts was measured spectrophotometricaly and the obtained results ranged from 56.62 mg/g to 172.50 mg GA/g. The antiproliferative activity of methanolic extracts from different Teucrium species was determined using MTT cell viability assay, where IC 50 value was used as a parameter for cytotoxicity. The type of cell death was explored by fluorescence microscopy using the acridin orange/ethidium bromide method. MTT assay showed that all extracts significantly reduced cell viability in a dose-dependent manner, with very low IC 50 values. The highest content of phenolic compounds and the best cytotoxic activity on HCT-116 cells after 24 h of exposure was in T. chamaedrys extract, with IC 50 values of 5.48 × 10 -9 μg/mL. After 72 h, methanolic extract of T. arduini appeared to have the best cytotoxic activity on HCT-116, with IC 50 values of 0.37 μg/mL. Treatments caused typical apoptotic morphological changes in HCT-116 cells and showed a high percentage of apoptotic cells. The results of the presented research indicate that some Teucrium extracts are a very rich source of phenols, which may directly contribute to high antiproliferative and proapoptotic activity. © 2011 by the authors; licensee MDPI, Basel, Switzerland. |
URI: | https://scidar.kg.ac.rs/handle/123456789/9667 |
Type: | article |
DOI: | 10.3390/ijms12074190 |
SCOPUS: | 2-s2.0-79960888620 |
Appears in Collections: | Faculty of Science, Kragujevac |
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10.3390-ijms12074190.pdf | 433.89 kB | Adobe PDF | View/Open |
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