Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/9667
Title: Teucrium plant species as natural sources of novel anticancer compounds: Antiproliferative, proapoptotic and antioxidant properties
Authors: Stankovíc M.
Ćurčić Milutinović, Milena
Žižić J.
Topuzovic, Marina
Solujic A.
Marković, Snežana
Journal: International Journal of Molecular Sciences
Issue Date: 1-Jul-2011
Abstract: This study deals with total phenolic content, antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and the effect on the prooxidant/antioxidant status in HCT-116 cells. The total phenolic content of the extracts was measured spectrophotometricaly and the obtained results ranged from 56.62 mg/g to 172.50 mg GA/g. The antiproliferative activity of methanolic extracts from different Teucrium species was determined using MTT cell viability assay, where IC 50 value was used as a parameter for cytotoxicity. The type of cell death was explored by fluorescence microscopy using the acridin orange/ethidium bromide method. MTT assay showed that all extracts significantly reduced cell viability in a dose-dependent manner, with very low IC 50 values. The highest content of phenolic compounds and the best cytotoxic activity on HCT-116 cells after 24 h of exposure was in T. chamaedrys extract, with IC 50 values of 5.48 × 10 -9 μg/mL. After 72 h, methanolic extract of T. arduini appeared to have the best cytotoxic activity on HCT-116, with IC 50 values of 0.37 μg/mL. Treatments caused typical apoptotic morphological changes in HCT-116 cells and showed a high percentage of apoptotic cells. The results of the presented research indicate that some Teucrium extracts are a very rich source of phenols, which may directly contribute to high antiproliferative and proapoptotic activity. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
URI: https://scidar.kg.ac.rs/handle/123456789/9667
Type: journal article
DOI: 10.3390/ijms12074190
SCOPUS: 79960888620
Appears in Collections:Faculty of Science, Kragujevac

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