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Title: New 4′-(4-chlorophenyl)-2,2′:6′,2″-terpyridine ruthenium(II) complexes: Synthesis, characterization, interaction with DNA/BSA and cytotoxicity studies
Authors: Ćurčić Milutinović, Milena
Rilak, Ana
Bratsos, Ioannis
Klisuric, Olivera
Vranes, Milan
Gligorijevic N.
Radulović M.
Bugarčić Z.
Journal: Journal of Inorganic Biochemistry
Issue Date: 1-Apr-2017
Abstract: © 2016 In this study, we have developed a series of new monofunctional Ru(II) complexes of the general formula mer-[Ru(Cl-Ph-tpy)(N-N)Cl]Cl in which Cl-Ph-tpy is 4′-(4-chlorophenyl)-2,2′:6′,2″-terpyridine, N-N is a bidentate chelating ligand (1,2-diaminoethane (en, 1), 1,2-diaminocyclohexane (dach, 2) or 2,2′-bipyridine (bpy, 3)). All complexes were fully characterized by elemental analysis and spectroscopic techniques (IR, UV–Vis, 1D and 2D NMR). Their chemical behavior in aqueous solution was studied by UV–Vis and NMR spectroscopy showing that all compounds are relatively labile leading to the formation of the corresponding aqua species 1aq–3aq. Their DNA binding ability was evaluated by UV–Vis spectroscopy, fluorescence quenching measurements and viscosity measurements. Competitive studies with ethidium bromide (EB) showed that the complexes can displace DNA-bound EB, suggesting strong competition with EB (Ksv = 1.1–2.7 × 104 M− 1). These experiments show that the ruthenium complexes interact with DNA via intercalation. The complexes bind to serum protein albumin displaying relatively high binding constants (Ksv = 104–105 M− 1). Compound 3 displayed from high to moderate cytotoxicity against two cancer cell lines HeLa and A549 (with IC50 ca. 12.7 μM and 53.8 μM, respectively), while complexes 1 and 2 showed only moderate cytotoxicity (with IC50 ca. 84.8 μM and 96.3 μM, respectively) against HeLa cells. The cell cycle analysis (by flow cytometry) of HeLa and A549 cells treated with complex 3 shows minor changes on the cell cycle phase distribution.
Type: Article
DOI: 10.1016/j.jinorgbio.2016.10.001
ISSN: 01620134
SCOPUS: 85009084375
Appears in Collections:Institute for Information Technologies, Kragujevac
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