Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/11769
Title: Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline
Authors: Radovanović, Mirjana
Jugovic, Branimir
Gvozdenović M.
Jokić B.
Grgur, Branimir
Bugarski-Vojinović M.
Knezevic Jugovic, Zorica
Journal: Starch/Staerke
Issue Date: 1-May-2016
Abstract: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. The immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability.
URI: https://scidar.kg.ac.rs/handle/123456789/11769
Type: Article
DOI: 10.1002/star.201500161
ISSN: 00389056
SCOPUS: 84949255495
Appears in Collections:Faculty of Agronomy, Čačak
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