Please use this identifier to cite or link to this item: https://scidar.kg.ac.rs/handle/123456789/13744
Title: Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones
Authors: Joksimović, Nenad
Milović, Emilija
Djordjic Crnogorac, Marija
Petrovic, Nina
Stanojkovic, Tatjana
Milivojević Dušan
Janković, Nenad
Issue Date: 2021
Abstract: In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.
URI: https://scidar.kg.ac.rs/handle/123456789/13744
Type: article
DOI: 10.1016/j.cbi.2021.109647
ISSN: 0009-2797
SCOPUS: 2-s2.0-85114842840
Appears in Collections:Faculty of Science, Kragujevac
Institute for Information Technologies, Kragujevac

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