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Title: Estimation of forces on actin filaments in living muscle from x-ray diffraction patterns and mechanical data
Authors: Mijailovich S.
Prodanovic, Momcilo
Irving T.
Issue Date: 2019
Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Many biological processes are triggered or driven by mechanical forces in the cytoskeletal network, but these transducing forces have rarely been assessed. Striated muscle, with its well-organized structure provides an opportunity to assess intracellular forces using small-angle X-ray fiber diffraction. We present a new methodology using Monte Carlo simulations of muscle contraction in an explicit 3D sarcomere lattice to predict the fiber deformations and length changes along thin filaments during contraction. Comparison of predicted diffraction patterns to experimental meridional X-ray reflection profiles allows assessment of the stepwise changes in intermonomer spacings and forces in the myofilaments within living muscle cells. These changes along the filament length reflect the effect of forces from randomly attached crossbridges. This approach enables correlation of the molecular events, such as the current number of attached crossbridges and the distributions of crossbridge forces to macroscopic measurements of force and length changes during muscle contraction. In addition, assessments of fluctuations in local forces in the myofilaments may reveal how variations in the filament forces acting on signaling proteins in the sarcomere M-bands and Z-discs modulate gene expression, protein synthesis and degradation, and as well to mechanisms of adaptation of muscle in response to changes in mechanical loading.
Type: article
DOI: 10.3390/ijms20236044
ISSN: 1661-6596
SCOPUS: 2-s2.0-85076048591
Appears in Collections:Institute for Information Technologies, Kragujevac

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