Novi zlato(III) kompleksi: Sinteza, karakterizacija i ispitivanje interakcija sa 5’-GMP, GSH i L-Met

Abstract

The pharmacologic properties of gold compounds have been known since the end of 19th century. They have been used for different studies, even though they are usually used for the treatment of rheumatoid arthritis. In the last decade gold complexes have received increased attention due to the variety of their applications. Primary, they have been investigated as potential anticancer and chemotherapeutic agents.1 It is well known that gold(III) complexes are very similar to platinum(II) compounds, so they could exhibit prospective anticancer, cytotoxic and antitumor properties.2 In this study we have synthesized two new gold(III) complexes with general formula [Au(N-N)Cl2] in which N-N is a bidentate ligand (4,7-diphenyl-1,10-phenanthroline and 2,9-dimethyl-1,10-phenanthroline). These complexes were characterized by spectroscopic techniques (IR, UV-Vis, 1H NMR). Kinetic of the substitution reactions between complexes and biological important molecules, such as guanosine-5’-monophosphate (5’-GMP), glutathione (GSH) and L-methionine (L-Met), were performed by stopped-flow technique. Also, we performed DNA binding studies using UV-Vis spectrophotometry, fluorescence spectroscopy and viscosity measurements, as like as interaction with bovine serum albumine (BSA).